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Alternatively, mutations could be generated in the terminal octomer through a slippage effect during replication.
This could imply that the binding of ubiquitin to the E3 is strong enough to promote polyubiquitination, yet loose enough to allow chain growth, possibly through a slippage mechanism by which the ubiquitin-binding surface specifically binds to the distal ubiquitin at the end of the chain.
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It thus seems likely that it is the tandem arrangement of ZNF-encoding motifs, per se, that confers a propensity for ZNF indel generation, possibly through a replication slippage mechanism (Krebs et al. 2005).
Using a combination of structural insight and pre-steady-state kinetics, it was recently shown that both Dpo4 and hpol κ generate single-base deletions on specific repetitive sequence mutational hot spots through a template slippage mechanism in which the template misaligns with the primer strand prior to nucleotide incorporation.
As shown in Figure 4A C and Table 1, full strand slippage (indicated by a slippage coordinate value of 1) accompanies unstacking of all three cytosines through either the minor (5° to 90° to 180°) or the major grooves (180° to 270° to 5°).
Was there a slippage in marking rigour?
"There could be a slippage of maybe a couple of weeks," said P. J. Crowley, a Pentagon spokesman.
It's been clear for a while that a slippage had developed".
"When a slippage occurs, the public is more cautious about giving to that organization".
"I don't know if that was a slippage of the tongue or what.
A few years ago, the Danes witnessed a slippage of their 4th graders' PISA math scores.
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