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Culture filtrates was treated by rotary evaporation under vacuum and then extracted thrice with a threefold of the volume of 95%(v/v) ethanol.
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Tumour size was measured thrice weekly using callipers and the volumes calculated as 0.52× a× b, where a and b were the minor and major tumour axes.
The cell biomass was washed thrice with one volume of sterile distilled water.
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