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For strains with severe locomotory defects, thresholds were adjusted on a case-by-case basis.
The voxel-level and cluster size thresholds were adjusted on an individual basis to avoid enlarged sulci near intact tissue being classified as lesion.
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Briefly, brown thresholds were adjusted to correlate with the immunopositive signal, whereas blue thresholds were adjusted for the nuclear counterstain.
The analyzer threshold was adjusted on the forward light scatter (FSC) channel to exclude most subcellular debris.
The analyser threshold was adjusted on the forward scatter channel to exclude the majority of subcellular debris.
As a common rule, the thresholds were adjusted based on the classification confidence values to evaluate the trade-off between FAR and FRR.
The thresholds were adjusted based on background signals in the central brain region.
Significance thresholds were adjusted using the Bonferroni correction.
Colour thresholds were adjusted accordingly, and the data generated in both instances was a percentage of brown intensity score based on the region examined.
Finally, for the Oil red O analysis, the colour density threshold was adjusted to distinguish all the fat droplets on a single fibre.
The thresholds are adjusted annually to keep up with the Consumer Price Index (CPI) + 1 percent, but not medical inflation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com