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However, standard evaluations of large, multi-dimensional gene expression datasets need to apply very strict statistical thresholds to correct for family wise errors stemming from the very high number of multiple comparisons.
From this, we provide a series of suggested Bonferroni p-value thresholds to correct for the multiple-testing burden in different populations, when using these arrays and imputed datasets.
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Because a preliminary analysis indicated no markers surpassed this threshold, no further effort was made to better define the statistical threshold to correct for multiple testing.
Results were then thresholded to correct for multiple comparisons at a cluster-level FWE rate of P< 0.05 (cluster-forming threshold: P< 0.001 at voxel level).
Subsequences that differ by one in the number of defined bases are also compared to each other using a threshold to correct for the higher expected count of the subsequence that contains lower number of defined bases.
Considering that satellite elevation can be treated as quality factor of observables, we build a model to calculate the standard deviations of the selected combinations and 20 degree can be used as threshold value to correct cycle slips or not.
As transgene expression was restricted to the injected side in all animals, we used the uninfected contralateral hemisphere as well as EGFP transduced sections to set the lower threshold value to correct for non-specific background.
Guthrie & Buchwald (1991) describe a threshold criterion to correct for sampling autocorrelation in waveforms but we could not employ that method because our initial response was too short and of low amplitude due to its high frequency.
Thresholds were set to correct for nonspecific antibody binding or fluorescence.
Other possible explanations include variable thresholds among clinicians to correct the commonly observed increased clotting times induced by DrotAA, or attempts to correct a bleeding event or a sepsis-induced coagulopathy.
Because heteroscedasticity resilient to transformation precluded the use of analysis of variance, a total of 22 t-tests were performed and P-value thresholds were established to correct for multiple comparisons as described by Benjamini and Hochberg [ 31] using the script published by IBM for SPSS software [ 32].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com