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CGIs are defined as clusters of CpG dinucleotides above particular thresholds of length, CpG frequency (corrected for GC content) and GC content (Fig. 4B) [16].
Furthermore, thresholds of length of stay were determined.
We were surprised to find salient contrasts in thresholds of length of stay and reimbursement.
We used independent sample t-tests to compare the thresholds of length of stay and the reimbursement of the different reconstructive options.
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The upper threshold of length of stay (uLoS) is the only trimpoint available in all countries of our series and is shown in Table 2.
The reads were filtered using Trimmomatic to remove adapters, FASTX toolkit and Sickle program to remove low quality reads and discard reads based upon the threshold of length.
Only query domain instances meeting the stringent threshold of length difference ≤ 20% and sequence similarity ≥ 50% are kept for the following analysis.
These results highlight the effects that the accumulation of prelamin A exerts on telomeres length, and specifically to shorten telomeres when they reach a minimum threshold of length in hMSCs.
Once the optimal alignment of the read is found, based on the costs specified above, a default filtering threshold of length fraction = 0.5 and similarity = 0.9 is applied to determine whether this match is good enough for the read to be included in the output and is further computed as average coverage.
The threshold of the FASTQ quality score was set at 30; the thresholds of the length of the trimmed reads were 60 bp for the single-end library and 50 bp for the two mate-paired libraries.
In Figures 4 and 5, CWT's ROC curves is limited in small FDR because two thresholds of the length of ridge lines and the scale corresponding to the maximum amplitude on the ridge line are used as default in MassSpecWavelet (Du et al., 2009).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com