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Peak coordinates of regions that showed connectivity with the PCC were identified for all analyses using cluster reports with the following thresholds: cluster size = 80 voxels (0.64 ml), minimum distance between peaks = 1 cm, and a BSR equivalent to a two-tailed p value of 0.001 (i.e., BSR = 3.3).
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By thresholding cluster sizes rather than pixel values to reject false positives, this approach enhances statistical power.
The original fraction of the 26 981 BF clusters among all clusters is a mere 0.5%, while the fraction following the minimal thresholds of cluster size >10 and LT >0.5 is 3.5%.
If the streaming capacity of a cluster complies to the threshold, the cluster size remains unchanged.
Measurements include tracking efficiency, charge sharing, time over threshold and cluster size distributions as a function of incident angle for IBL 3D design sensors.
This threshold and cluster size within a given ROI was chosen to help reduce noise, hence avoid the detection of spurious activations in isolated voxels.
Threshold correction was performed by using AlphaSim program (parameters were as follows: individual voxel p = 0.01, 1000 simulations, FWHM = 4 mm, with mask) in the REST software, which applied Monte Carlo simulation to calculate the probability of false-positive detection by taking into consideration both the individual voxel probability thresholding and cluster size [ 34].
The map was constrained using a voxel-level minimum statistic threshold and a cluster size threshold.
The group-level statistical parametric maps were constrained using a voxel-level minimum statistic threshold, and a cluster size threshold.
For each of the investigated SNP, voxel-by-voxel group comparisons were made using SPM99 (Welcome Functional Imaging Laboratory, London, United Kingdom) using proportional scaling, setting the height threshold at P≤0.001 and cluster size threshold at >100 voxels, and including group (C-LHPA vs. LPA) as a covariate.
The AlphaSim program provides an overall significance level achieved for various combinations of cluster size thresholds and probability thresholds for each voxel [ 30].
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