The percolation threshold of the twice-extruded poly acrylonitrile-co-butadiene-co-styrene)/CB compoly acrylonitrile-co-butadiene-co-styreneCB.
Hearts were either unpaced, or paced (as specified) at 5 Hz with 2 ms pulses of twice diastolic threshold via bipolar electrodes hooked onto the right atrial appendage.
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In the first above-quoted step, overexpression and underexpression for a given gene were assigned using a threshold of |1| corresponding to twice the expression level found in the normal breast pool.
The 2D analysis was performed in maximum intensity projections applying a threshold of mean intensity + twice standard deviation of a representative background region.
This used a bipolar, platinum-stimulating electrode (1 mm interpole spacing) placed against the right atrial epicardium, delivering square-wave stimuli (Grass S48 stimulator; Grass-Telefactor, Slough, UK) of 2-ms duration and amplitudes of twice diastolic excitation threshold.
The S1 S2 interval was initially equal to the pacing interval, then reduced by 1 ms with each subsequent cycle until S1 S2 = 6 ms. Both the WT and RyR2S/S hearts were stimulated using square-wave stimuli of 2 ms duration and amplitudes of twice diastolic excitation threshold (Sabir et al. 2007) (DS2A isolated constant voltage stimulator; Digitimer, Welwyn Garden City, Herts., UK).
To compare different origins, we divided the discrete slopes by their values at d = 0. We used the statistics of negative slope data to establish the error threshold (as twice the standard deviation of negative-slope data, fig. 4).
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Stimulation intensity was twice the threshold of the pudendal-anal reflex.
