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This miRNA is below the set threshold of expression in the inflorescences (Infl-Y; Infl-WD) and is expressed at higher levels (around 50 TP5M) in flowers at the beginning of flowering and in open flowers (Flower_FB and Flower_F); it targets genes annotated as encoding aphosphatidylinositol-4-phosphate 5-kinase, known to mediate pollen tube growth in Arabidopsis and Nicotiana tabacum [ 52, 53].
Core gene sets were analysed and using threshold of expression ≥ 20th percentile there were 16939 out of 17881 genes expressed in at least 1 cell line.
qPCR data were initially processed using the SDS1.2 software from Applied Biosystems to obtain Ct values, that is the amplification cycle number at which a reaction reaches a certain threshold of expression.
However, our modelling predicts that MCL-1 levels below a threshold of expression and not MCL-1 expression per se, should dictate resistance to BAD-like peptidomimetic induced apoptosis.
Another possible explanation would be that the repressive function of NRSF/REST requires a threshold of expression.
Strong viral promoters, such as the 35S cauliflower mosaic virus promoter, were thought to produce "aberrant RNA" after exceeding a certain threshold of expression [ 16, 85].
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This should entail adherence to NCI-EORTC recommendations for biomarker validation [ 45], requiring the incorporation of stringent quality control procedures, accurate quantification and scoring methods and unbiased thresholding of expression values.
The "normality" threshold of progastrin expression (low expression) was determined using the 95th percentile of percentage of stained cells in normal colonic tissue (<10%).
miRNA activity relies on a threshold level of expression [43], and certain miRNAs may not be expressed abundantly in certain cell types.
With all responsive cell lines, the quantities of response were comparable to those induced by LAN-5, regardless of GD2 expression levels, suggesting a threshold of GD2 expression necessary to trigger cytolytic T-cell responses via CARs.
Cells chosen to be precursors sustain high threshold levels of expression and signal to the cells surrounding them causing them to gradually lose proneural gene expression [1].
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