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Correctly, the pain threshold is defined at a perceptional level as the least experience of pain which a subject can recognize.
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In many studies, this threshold is defined as at least weekly reflux symptoms [ 2].
The anaerobic threshold (also referred to as the first ventilatory threshold) was determined by review of the gas exchange curves; the anaerobic threshold is defined as the oxygen uptake at which the ventilatory equivalent for oxygen increased systematically without an incremental change in the ventilatory equivalent for carbon dioxide [ 20].
The force is applied to the plantar surface of the mice hind paw, and the nociceptive threshold is defined as the force, in grams, at which the mouse withdraws its paw [ 22].
During the short sequence measurements, the cuff will be inflated at a rate of 1 kPa/s to determine the following variables: (1) pain threshold: the pain threshold is defined as the pressure of the cuff at the moment of transition from a sensation of strong pressure to the first sensation of pain (unit kPa).
Threshold was defined as the intensity at which the mouse would respond in 50% of the trials.
Pressure pain threshold was defined as the pressure at which the sensation changed from pressure alone to a combination of pressure and pain.
The threshold was defined as the point at which the second derivative exceeded 50% of its maximal value.
Action potential threshold was defined as the point at which the first temporal derivative of the voltage (dV/dt) first exceeded 55 mV/ms.
Action potential threshold was defined as the point at which the derivative of the membrane potential dV/dt deviated from the mean baseline value by >2 standard deviations [46], and was calculated using routines developed in our laboratory (Figure S2).
Action potential threshold was defined as the voltage at which dV/dt was >10 mV/ms.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com