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The digital images were processed using Image J (NIH), with an additional threshold color plug-in to process.jpeg images.
ImageJ software with threshold color plugin was used to quantify the area of interest that was immunoreactive for pIRE1 α, pPERK and AT8 antibody.
The area (mm) of positive MMP-2 expression was measured using a plug-in called "threshold color" from ImageJ software [25].
Then, we used the ImageJ software to adjust the threshold color of the subimage, until the contour of the ablation zone was well detected.
The percent area stained was quantified with ImageJ software using the threshold color function in RGB color space with pass thresholds set at red: 0 255, green: 0 104, and blue: 0 64.
By using the threshold color tool in the NIH Fiji©program, the areas stained in green were selected and in that way we could indirectly calculate the area fraction of collagen in the dermis.
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For "enlarged B", a specific pattern occurred in a window <40 ms, in 15 out of 50 trials (Figure 2B, Thresholded Color Sequences, middle, inset).
The "rotated A" stimulus elicited a specific pattern in 38 out of 50 trials but in a broader temporal window and in association with a few other patterns (Figure 2B, Thresholded Color Sequences, bottom, inset).
The most specific pattern for stimulus "Z" occurred only within a narrow temporal window of <17 ms (Figure 2B, Thresholded Color Sequences, top, inset) in 16 out of 50 trials.
Categorical color perception is one of the supra-threshold color perceptions, but it represents colors in a symbolic and discrete manner.
Easy Leaf Area uses a combination of thresholding, color ratios, and connected component analysis to rapidly measure leaf area in individual images in seconds or batch process hundreds of images in minutes; results are saved to a spreadsheet-ready CSV file.
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