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The first step recognized highly correlated metabolites through the construction of a "union network" based on a Spearmann rank-order analyte-analyte correlation in each cultivar for the 123 identified analytes, which was transformed into binary matrices according to a p<E−4 threshold (applying Bonferroni correction) and considered further if it was significant in at least one cultivar.
As we considered 2413 independent SNPs in the candidate loci for these metabolites, the statistical threshold, applying Bonferroni correction at a nominal p-value of 0.05, for the replication study was 4.06E-07 (0.05/2413*51).
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It is the highest threshold applied in Europe, and at the same it makes the project a reliable one.
c Threshold applied.
Fig. 1 Blanking nonlinearity at very low fixed threshold applied to IN-affected signal Fig. 2 Blanking nonlinearity at very high fixed threshold applied to IN-affected signals.
The FPR, TPR, and TNR values vary with the threshold applied for classification (see (2)).
The threshold applied to each telescope sensor is a critical parameter for the telescope performance.
The threshold applies whether you are single, or married and filing jointly; married people filing separately cannot convert at all.
In addition to the performance characteristics just described, we also examined the issue of falsely predicted proteins, as a function of the threshold applied to posterior probabilities.
The existence of "looped-out" chromosome regions implies the threshold applied is generally too severe, with the edge defined to close to the chromosome centroid.
This observation clearly supports the robustness of our predictions and justifies the selection of the particular bitscore threshold applied in this study.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com