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The low-threshold accessibility of the Internet makes it suitable for offering and receiving help for psychological problems.
The default threshold of accessibility is set to the highest value that observed for all 125 validated MTIs recovered by imiRTP.
This measure overcomes the well-known problems with the arbitrary selection of time thresholds and extreme sensitiveness of small travel time changes associated with threshold-based accessibility measures [29].
We filtered the results of this exploration to keep only the pairs located on the protein surface as it is the most accessible location for known therapeutic targets (accessibility threshold greater than 25%, using the ASA software [ 26] and implemented by Allan et al. [ 27] based on the 3D PR structure PDB ID 1HSG [ 28] and 3D RT structure PDB ID 1DLO [ 29]).
All AAs having an accessibility threshold greater than 25% are considered "accessible".
For the threshold of solvent accessibility, we used a cut-off of 7.0% relative total side-chain accessibility.
The effect of this threshold on the accessibility index is small, however.
For a relative accessibility threshold of 25%, only 46.1% of PPIIDSSP are buried while in the case of turns, β-strands, α-helices and coils, 35.8%, 72.2%, 55.4% and 51.9% of residues are buried, respectively.
A solvent accessibility threshold of 25% was used for classifying residues as exposed or buried.
It implies that C2 crosses a new threshold of C4 accessibility, and that C4 is all but an inevitable outcome.
However, these results also suggest an accessibility threshold, above which enzymatic digestibility does not depend heavily on further pore size expansion.
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