The dispersions were further diluted using a threefold dilution method in glucose stock in such a manner that the second through the seventh rows of wells contained sample dispersed in concentrations of 3.33%, 1.11%, 0.37%, 0.12%, 0.04%, and 0.01%.
For example, the early Church Fathers sometimes used a threefold (later fourfold) method of interpreting texts, encompassing literal, moral, and spiritual meanings.
This method is threefold: experimental, numerical, and comparative.
The advantage of our method is threefold.
We developed a highly sensitive probe-free quantitative mutant-allele detection method, Quantitative Threefold Allele-Specific PCR (QuanTAS-PCR), that is performed in a closed-tube system, thus eliminating the manipulation of PCR products.
The advantages over ad hoc heuristic methods are threefold: (i) having provably optimal solutions at hand allows evaluating the quality of a model, e.g. the appropriateness of an objective function.
The goals of this paper are threefold: 1) to review computational methods and to update graphs for estimating aS0, 2) to survey current information about aS0 in modern sulfidic water bodies and 3) to explore briefly what this information might imply about biogeochemical processes.
We also tested a new density gradient centrifugation system, able to enrich the tumor fraction of BM specimens by twofold to threefold as compared with standard Ficoll methods.
The contribution of our paper is thus threefold: We adapt four different metaheuristic methods to the special requirements of the DSDARP (VNS, S-VNS, MPA, and MSA) and identify problem characteristics for which one or the other method works better.
The new algorithm can give a threefold increase in overall speed over existing methods with the same order of accuracy, or give greater accuracy for the same computational effort; the accuracy decreases slowly as c increases beyond unity.
