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Receptor-attached phosphates destabilize both the polar core (Vishnivetskiy et al., 1999) and three-element interaction (Vishnivetskiy et al., 2000), enabling transition into high-affinity receptor-binding state.
The other clasp is the three-element interaction of β-strand I and α-helix in the N-domain, and β-strand XX in the C-tail (Hirsch et al., 1999) (Figs. 1 and 2A).
Note that β-strand XX, anchored to β-strand I and -α-helix via three-element interaction (see Fig. 2A), includes virtually the whole part of the C-tail resolved in crystal structures Open image in new window Figure 2 Localization of key functional elements in arrestins.
Scanning mutagenesis of the whole arrestin-1 molecule (Ostermaier et al., 2014) confirmed earlier findings regarding the role of the polar core and three-element interaction in maintaining the basal arrestin conformation, as well as the role of Arg18 and Lys20 in phosphate binding (Hanson and Gurevich, 2006).
A second feature maintaining the basal conformation is the "three-element interaction" of hydrophobic residues in beta strand I, alpha helix I, and beta strand XX in the C-terminal tail [ 25].
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A critical challenge now is to extract from more detailed orbital descriptions, with millions of interaction elements between orbitals, predictions of how primary sequence and folding-induced contacts influence electron transfer rates.
A proposed solution : Justiciability Council and four-element justiciability test.
For example the 4th element of the 1st thread is mapped via the 4th element of row 1 of the Save Structure to location 384 in shared memory.
Compendium: The 47th element on the Periodic Table.
Enhancement of ion/neutral 1st wall interaction with 2nd SOL and saturated wall is discussed as an important R&D element for DEMO and existence of high-energy neutrals in JT-60U was mentioned.
MEASUREMENTS: Audiotapes were analyzed to characterize communication style and coverage of the standard elements of informed consent and, using the Roter Interaction Analysis System, to capture the dynamics of three-way interaction between the patient, their companion, and the physician investigator.
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