After working for the camps for three wks., she quit & married a reporter.
Three wks after DNA vaccination rats were injected s.c. in the base of the tail with 200 µl inoculum containing 1∶1 100 µg MOG91 108 in PBS emulsified in CFA, consisting of IFA (Sigma, St Louis, MO) and 0.5 mg heat-inactivated Myobacterium tuberculosis (H37 RA strain, Difco Laboratories, Detroit, MI).
Two wk after weaning, calves were blocked by age and randomly assigned to 4 treatments according to a randomized complete block design.
Twenty-four Ile de France ram lambs were evaluated in a 2 × 2 factorial design involving different DL-α-tocopheryl acetate injections (C, control = 0 and V = 150 IU/wk, i.m. for eight wk) and energy level (N, normal = 7.61 and L, low = 6.47 MJ/kg DM).
Eight wk old C57BL/6 mice were purchased from the Jackson Laboratories.
Rat splenocytes were isolated from five-to-six wk old female DA rats.
Four-to-five wk olocallylly bred LEW.1AV1 (RT1av1) or DA (B&K, Sweden) female rats were used in the experiments.
Cells were cultured in DMEM supplemented with 10% heat inactivated fetal calf serum, 1% pencillin-streptomycin and 1% L-glutamine (all from Invitrogen) for 24 h at 37 C. Four-to-five wk oLEW.1AV1AV1 or DA female rats were injected with 100 µl of 10 µM cardiotoxin (Latoxan, Rosans, France) in the gastrocnemii muscles.
Increasing returns to scale exists if the value of wk is greater than zero (wk > 0), constant returns to scale if the value of wk is equal to zero (wk = 0), and decreasing returns to scale if the value of wk is less than zero (wk < 0).
It is the average of three 11-point numerical rating scales pain today, worst pain in the last 4 wk, and average pain in the last four wk.
