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Confluence (≥80%) and homogeneity of the culture were obtained after approximately three week of cell culture.
Based on the size of spheroids recommended by manufacture´s (± 300 μm) we found out that 1 × 10 cells/well and three week of observation was sufficient to produce 20 (± 4) spheroids/well with size of 284,12 μm (± 40,21) as shown in Figure 6B.
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Three weeks of long nights later, I launched Muxtape.
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