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An active structure of the TSHR TMD (TMD_Ac) was built based on the only three templates of fully active GPCRs.
qPCR was performed in triplicate with, at least, three templates of RNA extracted from independent cultures.> The data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus [ 51] and are accessible through GEO Series accession number GSE60107.
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When combined with immunocytochemical detection of proteins, ISH was performed at lower temperature (52°C), lower salt concentration (SSC 1,3×), and pH = 5.0) or alternatively, using DNA probes that were generated by PCR using two templates of the cDelta1 cDNA (678 914 bp and 1085 1425 bp: Access N° U26590), and of Tis21 as previously described [46].
Four templates of PLND have been well defined: limited, standard, extended and super-extended.
Two templates of DES models, for PHCCs and FHUs, were designed and implemented in the Simul8 computer software package (version 13.0) [ 26].
A total of three templates were shortlisted and their secondary structure alignments are shown in Table S9.
In the reference dataset, the FPCN is represented by two templates, consisting of the left and right hemisphere regions of the FPCN.
The training image is scanned four times using four square templates of different sizes including 17, 21, 25 and 29 grids on each edge.
Taken together, these results indicate that T7 DNAP binds to three template-bases downstream of primer-end and melts two base pairs.
While each of these two process templates specifies a single kinetic law, the two process templates of GAPProcess and GAPProcessPlus specify two and three alternatives for modeling the hydrolysis of GAP7 and GAP5 respectively.
Assuming amplification of two template copies of genomic DNA from the control region, the relative copy number for each target region was calculated as 2∧(1+ (-ΔΔct)).
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