For the multi-objective cost-efficiency evaluation (IPOG-F, jenny), we followed the same two steps previously mentioned: transformation of the cost-efficiency (two-dimensional) representation into a one-dimensional one and usage of statistical tests, such as the t-test or the nonparametric Wilcoxon test (Signed Rank) (Kohl 2015), to compare each pair of test suites (TTR 1.2 and other).
Each state is an ordered triple of the current and past two cues, e.g. (A,B,C) if cue A was followed by cue B and then cue C. When generating sequences, a given cue can not occur twice in a row, so for any one of the 4 observations, there are 3 observations that can precede it and 3 possible observations that can occur two steps previously, for 36 possible states.
Finally, the NheI/blunt/ NotI fragment from pTYF-1 × SYN-EGFP or pTYF-1 × GfaABC1D-EGFP was inserted into the resultant plasmid from the above two steps previously treated with MluI/blunt/ NotI to produce pTYF-mCMV/SYN-EGFP and pTYF-mCMV/GfaABC1D-EGFP respectively.
Screening was performed in three steps as described previously [60].
Reagents and instrumentation: The Boc-protected PNA backbone methyl ester, bearing an l-Arg residue with tosyl protection in the side chain (Boc- 5l-Arg(Tos -PNAbackbone-OMe) was prepared from commercial Boc- l-Arg(Tos -PNAbackbone-OMeps, as reporTos -PNAbackbone-OMe
The double immunocytochemical method was performed in two steps as previously described [ 21].
The selectivity test was composed of two steps as previously reported for the validation of qPCR methods for food pathogens (Barbau-Piednoir et al. 2013a, b).
The resulting protected target molecule 4 was then deprotected in two steps using previously published procedures to give 1. in high yield.
The t Bu-protected PSMA binding motif ((OtBu KuE(OtBu 2, 3) was synthesized in 73% yield via three steps of solution phase chemistry as previously described with minor modifications (Figure 3, [12]).
The tBu-protected PSMA binding motifs (OtBu EuE(OtBu 2 and (OtBu KuE(OtBu 2 were synthesized in 84 and 92% yield via three steps by solution phase synthesis as previously described [17].
