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Induction of the dsRNA resulted in a 50% decrease in the TbMyo1 transcript within 24 h as indicated by the relative expression ratio (induced/noninduced) of 0.5±0.1 determined by qRT-PCR (mean ± SD of three separate determinations).
Induction of the dsRNA resulted in a 90% loss of the TbMyo1 transcript within 24 h as indicated by the relative expression ratio (induced/noninduced) of 0.11±0.06 determined by qRT-PCR (mean ± SD of three separate determinations).
Each result is the average of three separate determinations.
[25] Each result is the average of three separate determinations.
Relative standard deviation of three separate determinations by the method was found to be 5.6%.
On the other hand, for real human plasma, the percentage recoveries based on the average of three separate determinations were 92.23 ± 2.89.
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The percentage recoveries are based on the average of four separate determinations.
The percentage recoveries based on the average of four separate determinations were 92.80 ± 2.27.
The percentage recoveries based on the average of five separate determinations were 101.05 ± 1.65, thus indicating the high precision of the method (Table 4).
Quantitative analysis of three to four separate determinations is depicted in Fig. 7B (calcineurin) and Fig. 7C (NFAT) normalized to GAPDH.
Four separate determinations were carried out on each sample; means and standard deviations are given.
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