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Three restriction endonucleases, that is AseI, DraI, and XbaI were attempted in PFGE profiling.
The amplified products of DNA from 80 Cronobacter strains were separately digested with three restriction endonucleases (Csp6I, HinP1I, MboI).
The mentioned method was based on isolation of Trichostrongylus ova from human fecal samples using Willis method, the extraction of ova genomic DNA samples, followed by rDNA ITSII PCR and one-step multiplex RE-PCR using three restriction enzymes of HinfI, DraI, and MseI.
These rabies viruses were also amplified by RT-PCR with general rabies primers and the PCR products were cut by three restriction enzymes, Blp I, Bsu36 I and BspE I. Results: All the DRRV and VRRV were distinguished by RT-PCR with SS primers.
Because three restriction groups were not homogenous, we used alternative restriction endonucleases to distinguish the different subgroups.
Three restriction enzymes were predicted to generate single fragments with this probe.
Similar(6)
MNP model is free of the MNL model's three restrictions.
It is straightforward to show that, under three restrictions, (max{p} = 100) and (min{p} = -100).
"We had about 600 calls in the first five days of the level three restrictions".
This model has three restrictions, and hence 3 df.
Twelve restriction enzymes were tested on C. tropicalis strains.
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