Sentence examples for three overlapping fragments from inspiring English sources

The 5'-RACE generated three overlapping fragments of about 300, 450 and 500 bp.

Primers covering three overlapping fragments were used for reducing the likelihood that a nuclear insertion rather than the organelle mtDNA was amplified [23], [28], [29].

As a result, three overlapping fragments gave rise to one unique agrin sequence instead of the two ORFs predicted by WormBase.

Three primer pairs were designed from conserved regions of primates, rodents, cow and dog and used to amplify three overlapping fragments (see Table S2, Supplementary Material online).

For analysis of the NcrB binding site, the sequence of the promoter region (n32p43) was divided into three overlapping fragments (n32p6, n16p22, and p7p43), as shown in Fig. 3B.

To further delineate the binding regions for DAF-16 and HSF-1 binding within the promoter fragments in pWBI084/085, we subdivided this 160-bp fragment into three overlapping fragments of 60 65-bp 60 65-bp

Six overlapping fragments covering the initially identified 62 kb candidate region (Figure3a) were picked from a newly developed sub-library of a Koshihikari BAC clone.

The complete MgPa operon was amplified in up to six overlapping fragments from all specimens studied (Table 1).

The entire coding and proximal promoter regions of the CYP21A2 gene were specifically amplified in four overlapping fragments using primers already described [14] [16].

This was achieved through the amplification of five overlapping fragments of 173, 177, 119, 119 and 119 bp respectively, using a two-step PCR protocol [33].

The CYP21A2 gene and the closest 5' and 3' UTR were sequenced after being amplified in two overlapping fragments by the polymerase chain reaction method as previously described [18].