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WHO had recommended the three lineage strains for 2006 2009, the time perod during which the strains had been circulating.
We performed three lineage experiments on mice called ML2, ML4 and ML7 at the age of 10, 13 and 5.5 weeks respectively.
We reconstructed three lineage trees of cells of multiple types obtained from three adult mice based on the analysis of spontaneous somatic mutations accumulated in microsatellites.
Levels of both H4K16ac and H4K8ac were lower at the promoter of the constitutively active housekeeping gene Gapdh than at the promoters of the three lineage specific genes tested (Fig. 2, supplementary Fig. S1).
Staining of 20 clones for markers of neurons (TuJ1), glial cells (GFAP) and smooth muscle cells (SMA) revealed 12 clones (63%) positive for all three lineage markers, 3 clones (16%) positive for TuJ1 and GFAP, 3 clones (16%) positive for SMA only, and 1 clone (5%) positive for TuJ1 only (Figure 8).
Day 20- Three lineage tracing alloxan plus caerulein mice, three lineage tracing alloxan alone mice, seven C57BL/6 alloxan plus caerulein mice, seven C57BL/6 alloxan alone mice.
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The remaining three lineages; however, are morphologically homogeneous and may represent cryptic species.
The cells were able to maintain their potential for differentiation into the above three lineages.
A minimum spanning haplotype network also supported separation into three lineages.
Three lineages with distinct genotypic and antigenic characteristics have been identified from Chikungunya virus isolates collected from various geographical areas.
Finally, cardiovascular progenitors give rise to all three lineages of the heart: cardiomyocytes, endothelial cells, and smooth muscle cells.
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