Using the five WT models and the SCAP program, which is part of Jackal package (http://wiki.c2b2.columbia.edu/honiglab_public/index.php/Software:Jackal), we created separate structures for the three independent point mutations investigated in this work (R18K, L66H and R213W).
Taken together, these segregation data and, more importantly, the occurrence of the aberrant nodule phenotype in several independent mutation events (especially three, independent point-mutation lines) provides unequivocal evidence that functional SACPD-C is required for normal nodule development in soybean.
Graphene, a mono-layer of sp 2 bonded carbon atoms arranged in a honeycomb pattern (Figure 1a), is a two-dimensional semi-metal where the valence and conduction bands touch in two independent points at the border of the Brillouin zone, named K and K' valleys [1 5].
Hence each system was started from two independent points of the conformational spaces and provided independent sampling totalling to 60 ns, which we believe to be satisfactory for the purpose mentioned by the reviewer.
A distinct difference between Amber99 and Amber99+GBSA (in terms of their correlation with RMSD) can be observed, but the result has no statistical significance, as this correlation is computed over a set of five independent points only.
In this TILLING screen, we identified seven independent point mutations among which two led to L124F and G194D missense mutations.
TILLING of Sl-eIF4E1 yielded seven independent point mutations, which correspond to one silent, four intronic, one missense and one splicing site mutations (Table 3).
It was assumed that cases and controls formed two independent point-Poisson processes of different intensities.
We generated two independent point mutants in key residues of the catalytic site of Ccr4 (D558andnd H665A) [ 50].
