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Furthermore, three immunizations of C57BL/6 mice with 106 freeze-thawed mGC8 tumor cells at weekly intervals either prevented growth of subcutaneously injected live mGC8 cells completely or tumor outgrowth was delayed for nearly three weeks depending on the injected tumor cell dose (Fig. 7B, C).
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We show that two immunizations of modified non-replicating mRNA encoding influenza H10 hemagglutinin (HA) and encapsulated in lipid nanoparticles (LNP) induce protective HA inhibition titers and H10-specific CD4+ T cell responses after intramuscular or intradermal delivery in rhesus macaques.
Positive controls were CD4+ T cells isolated from animals prior sensitized with two immunizations of 3×107 donor splenocytes over 10 days.
In 1980, Tolpin et al. reported that two immunizations of mice with inactivated MCMV vaccine could provide a protection rate of 89% against a low-level virus challenge.
Over the course of three immunizations, only 8 of 315 (3%) of solicited systemic events were rated Grade 3, and these occurred in five volunteers (14% of volunteers); all were immunized with full dose AMA-1/AS01B.
In one of two monkeys given a series of three immunizations with an 8 mg dose of peptide, granulomatous inflammation was noted at necropsy at the injection site.
After three immunizations, the detection of specific antibodies in sera was performed by enzyme-linked immunosorbent assay (ELISA).
To determine the dose-range of FLU-NA-DIII able to induce protective immunity after two immunizations, groups of mice were immunized with a range of doses (101 106).
Laboratory tests were assessed on days 0, 2, 7, 14 and 28 relative to each of the three immunizations and also at the end of the study (week 52).
Mice received a total of three immunizations at two week intervals.
A total of three immunizations were given at one-month intervals, all administered intramuscularly in alternate arms.
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