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Our experiment compared the fitness (mating success, longevity, and productivity) of individuals possessing genomes that derived two generations previously from males that were either entirely successful (studs) or wholly unsuccessful (duds) at achieving mates in three subsequent rounds of mating trials.
The choice of this population was guided by the fact that it was the only population available to use which had recently been isolated from the field (two generations previously), that had been initiated by a large number of founders, and did not contain sex ratio distorters.
Tlr5−/− mice were derived and backcrossed to a C57Bl/6 background for eight generations as previously described [31].
The observed ratio of 9.60 1 is in agreement with the 7.98 1 ratio expected for a single recessive gene with a frequency of 0.125 after three generations of selfing from a previously random mated population (chi-sq 1df p-value 0.15), consistent with genetic control by a single recessive gene.
This mapping population, designated AL-NF, was developed from four founders in three generations and, as such, is unique from previously reported mapping populations in switchgrass.
mPGES-1-KO (knockout) mice were established as described previously [ 12, 13], and backcrossed at least three generations with C57BL/6 mice or ten generations with BALB/c mice.
Previously, we described a family with five individuals affected with Bipolar I disorder in three generations and one individual with recurrent unipolar depression [ 3].
Tip30 −/− Balb/c mice were obtained by backcrossing Balb/c mice with Tip30 −/− C57BL/6 mice seven generations as described previously.
Three generations back.
The problem spans three generations.
They were three generations from one family.
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