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Beyond that, the in vivo microenvironment can provide tissue specific factors and three dimensional context for cell survival, which may be even better than in vitro cell culture condition.
This model provides a useful tool to study keratinocyte and fibroblast responses in a three dimensional context which is more physiological than typical skin cell culture.
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These techniques may be useful in developing therapies for replacing lost tissue function, as in vitro models of living tissue, and also for further enabling fundamental studies of structure/function relationships in three dimensional contexts.
Examining evolution in the context of three dimensional structure adds power to the analysis in detecting additional positive selection and displaying the complexity of the selection process.
The MAME model allows for co-culture of multiple cell types in the context of a three dimensional microenvironment.
Since these structures were obtained with portions of proteins, it can simply not be informative in the context of the three dimensional arrangements of the full length protein.
We have emphasized in each individual case the gain from analyzing the coevolution data in the context of the three dimensional Rosetta-GREMLIN models over what could be gleaned from the co-evolution and conservation data alone.
A measure is simply a device to attribute a 'size' to a part of a space (in everyday contexts one, two, or three dimensional).
They now examine where these various blocks of conservation are in the context of key regions within the three dimensional structure of the ribosome.
However, three dimensional structures in the oligomeric form of AMPs in the context of lipid membranes are largely limited.
Modulation of gene expression in the context of intact pancreatic islets is particularly difficult because of their three dimensional structure (50-500 μm in diameter), making physical access to the islet core difficult.
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