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Each amplification was performed in duplicate using at least three different preparations of first-strand cDNAs prepared from each organ (n≥3).
Three different preparations and imaging techniques were used.
Comparisons among three different preparations denoted that in the investigated conditions two-step preparations give larger yields of activated solid in comparison with single-step preparation.
For mouse and rat tissues, each amplification was performed three times using three different preparations of first strand cDNAs resulting from three different RNA extractions.
All experiments were reproduced with at least three different preparations.
For rHSCs, experimental data arise from three different preparations of stellate cells from different animals.
Similar(44)
Nitrogen doped TiO2 materials were successfully prepared following three different preparation routes (sol gel, hydrothermal and pyrolysis) and characterized by various spectroscopic techniques.
Cells from liquid cultures were prepared for EM using three different preparation methods: conventional fixation and embedding, high-pressure freezing/freeze-substitution fixation, and plunge-freezing (Figure 1).
We found that there were significant differences in the size distribution of the amplified libraries when comparing the three different preparation protocols.
For this reason, three different preparation techniques were considered, i.e. sonication, ball milling and high-pressure homogenization.
Reactivity results obtained show expected reducibility, oxygen carrying capacity and stability with the three different preparation techniques.
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