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The inhibition constants were obtained by nonlinear least square methods using PRISM 3 (GraphPad Software, La Jolla, CA, USA), and they represent the mean from at least three different determinations.
Results are averages±SEM of three different determinations performed in duplicate.
Data are indicated as mean ± SD of three different determinations.
The mean values were represented from at least three different determinations [31,34].
The kinetic parameters for the uninhibited enzymes were derived from Lineweaver-Burk plots, as reported earlier [ 49– 51], and represent the mean from at least three different determinations.
IC50 values were obtained from dose response curves working at seven different concentrations of test compound; by fitting the curves using PRISM (http://www.graphpad.com/) and nonlinear least squares methods, values represent the mean of at least three different determinations as described by us previously [ 27].
Similar(54)
Incubation of confluent BHK-21 cells monolayers (as used for infections with FMDV) with 5 mM R for 31 hours resulted in cell viabilities of 40% to 50%, as determined with trypan blue exclusion (range calculated in six different determinations).
No significant differences were observed between plasma levels of α-defensins 1-3 in the two different determinations in patients that remained controllers (Fig. 5D, white boxes).
Five different determinations were used to develop the statistical data related to calibration curve (Table 1).
Statistical data of the calibration curve are obtained from five different determinations were shown in Table 1.
On Salmon's view, the existence of atoms can be inferred as the common cause of Perrin's thirteen different determinations of Avogadro's number.
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