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The intuition is that if two genes are co-expressed in the wild type and two deletion strains, they might also be co-expressed in the third deletion strain, given that the deleted genes in the three deletion strains are known to be involved in similar biological processes.
We generated three deletion mutants of TUT7 by deleting domains from the N-terminus of TUT7 (ΔNtr1 ΔNtr1, and ΔPAP1) (Fig 1A).
The results showed that fewer reducing sugar ends were released from the incubation of the supernatants from the three deletion strains when compared to the WT supernatant.
Statistical analysis showed that the result was significantly different to the WT strain for the xlnR deletion strain after 24 h and the results were significantly different to the WT strain for the three deletion strains after 48 h.
Mutant M5A and all three deletion mutants showed a dramatic loss of luciferase activity (Figure 4B).
In gene tonB, one insertion of two codons (isolate SB3336) and three deletion events (one of four codons, and two of two codons) were observed.
Similar(15)
A total of 14 probes were designed to detect the most frequently three deletions (-α3.7, -α4.2, –SEA) and two non-deletions (αQuong Sze, αConstant Spring).
However, only three deletions were reported to extend to either olfactory receptor region.
In addition, we have found three deletions, two in prenatal diagnosis (1 female and 1 male).
The new mutations include 5 translocation breakpoints, three deletions and nine other mutations.
Interestingly, three deletions did not yield transformants (GON7, UAF30 and OCH1).
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