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PBMCs were stimulated with anti-CD3 plus anti-CD28 for three days, subsequently pulsed with 60 μM bromodeoxyuridine (BrdU) (Sigma-Aldrich), and harvested 18 hours later.
Survival was scored as the number of successfully eclosed adults, measured two days after first observed eclosion (per strain/treatment combination) and three days subsequently.
In half these cases, the notion of an ER was recorded in the patient's preadmission notes.> On admission, the patient expressed an ER, repeated over three days, subsequently moving towards an expression of the WD.
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Six days subsequently, an email containing the survey link was sent out.
Subsequently, three days later, in their showroom, Stan saw 'The World of G.I. Joe," a full line of action figures and their appendages.
Subsequently, three days later we infected them with the fungal entomopathogen Beauveria bassiana by injecting conidia spores into the hemocoel.
Subsequently, colonization was assessed three days after infection (Fig. 2).
Three days later, MTT was added to the cells which were subsequently lysed after 1 h.
He subsequently went into shock and experienced organ failure, and died three days later, on April 1.
Roosevelt first used the phrase in a conversation at the Minnesota State Fair on September 2, 1901, four days before the assassination of President William McKinley who died an additional eight days later, which subsequently thrust Roosevelt into the presidency.
Subsequently, five days after rash onset, measles IgM and IgG became positive.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com