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In this study they evaluated the impact of three cultivation factors, amounts of refinery residue, glutamic acid and yeast extract.
The mean performance of the control lines (parental lines and F1 hybrids) across the three cultivation tests was evaluated.
Under shade-limiting conditions, both AFDW and Chl-a concentrations varied inversely with μ for the three cultivation methods.
Since five lines including the three heterokaryotic parental lines (Horst U1, WB2, WW7) and two F1 hybrids (Cr012, Cr002) were grown three times as control lines in three cultivation tests (Gao et al. 2015), their performances for all traits were calculated as the mean values over the three cultivation tests.
The CC of Horst U1, WW7 and Cr002 were consistently scored as "1" since they were white; WB2 and Cr012 were consistently scored as "3" (light brown) in all three cultivation tests.
Three cultivation substrates were tested using the following agricultural byproducts obtained from local markets: (1) Pangola grass (Digitaria decumbens) cut into 2 2.5 cm lengths, (2) corncob (Zea mays) cut into 1 2 cm lengths, and (3) a mixture of wood shavings of the primavera tree (Tabebuia rosea) (16.6%), with corncob (41.6%) and Pangola grass (41.6%).
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In present study, we conducted an outdoor 15N labeling pot experiment in Mollisols of Northeast China, where plants were consecutively cropped after each of three cultivations (spring wheat-buckwheat-spring wheat).
Process data of double knock out mutant is given in Additional file 3. Three cultivations were performed; the only difference between the first and the two last was the fructose isotopomer composition used in the C-labelled feed.
After three cultivations for 18 h in TSBY at 37°C, cultures were diluted to an OD600 of 0.05 for S. aureus and of 0.5 for C. albicans and P. aeruginosa.
The in silico method is provided in the Additional file 2. A representative growth curve from three cultivations was plotted against the simulated growth curve to evaluate the behavior of the model.
Hundreds of well-heeled investors and would-be farmers poured in from across the country to vie for four cultivation permits.
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