In contrast to the conventional polycations with long sequences of covalently bonded repeating units, this work reports a supramolecular gene carrier where many cationic cyclic units are threaded over a polymer chain to form a chain-interlock structured gene carrier.
A modified Kuntscher nail, of appropriate length, was threaded over the guide-wire and advanced until only a finger-breadth of the nail was palpable above the piriform fossa.
A well-lubricated, one-pass, long, tapered dilator was threaded over the guidewire into the trachea.
Southern and Knowles developed a single lumen polyethylene 10 tubing threaded over a 30-gauge needle acting both as electrode and perfusion line.
Hollow fiber cellulosic membrane (Filtral 12, AN69 HF, Hospal, 300 μm diameter) was cut to 10 mm and threaded over the silica tubing and inside the shaft.
Two 50 mm lengths of fused silica tubing (SGE Analytical Science, UK) with an outside diameter of 170 μm and an inside diameter of 110 μm were threaded inside the shaft with one threaded over the inlet and outlet tubes, respectively, to form a Y-shaped assembly.
When the chicken is flipped, you should see the twine bends threaded over each thigh and under each wing.
Tighten the connection with a wrench, taking care not to cross-thread or over-tighten them.
