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The amplification product contained thousands of repeated sequences which could hybridize with the loop of molecular beacons (the detection probes), resulting in a significant fluorescence signal.
They obtained a RCA product with thousands of repeated sequences of a long single-stranded DNA which was exploited for linear periodic hybridization when utilizing the Qdot detection probe.
The amplified synthesis was achieved by combining a target DNA related ligase reaction with rolling circle amplification (RCA), which produced thousands of repeated sequences to bind hemin and form a mass of horseradish peroxidase-mimicing DNAzyme units.
For example, this phenomenon occurs in DM1 and DM2, which are caused by several hundreds or thousands of repeated units present in the transcripts.
Our ELRCA design has the advantage of exponential amplification and the production of thousands of repeated sequences under isothermal conditions with high efficiency and specificity.
Using RCA, we initially generated self-assembled composite DNA microsponge structures containing ODN's in the form of long single-stranded oligonucleotide (ssDNA) polymers that each contain several thousands of repeated ODN copies complementary to a selected target sequence for antisense therapy.
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For example, compare the 1st, 2nd and 20th s of repeated stimulation at bright luminance level (Fig. 2C).
Three different generations (1st, 2nd and 3rd) of repeated recycled concrete were produced using 100% recycled coarse aggregate (RCA) as a replacement of natural coarse aggregate where RCA was recycled 3 times over its life span.
Textile printing, however, was known in Europe in the 6th century, the designs consisting largely of repeated decorative patterns.
But many more of the storied building's occupants fit the mold of the budget analyst on the 13th floor, a 28-year survivor of repeated central-office shake-ups, whose mission is to ensure that everything from snacks to teachers is allocated fairly to 1,100 schools in 32 districts.
Fig. 1 Scatterplot of repeated SUVmax measurements of 60-min scans for 45 patients (blue: 1st reading, red: 2nd reading).
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