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To directly analyse autophagic clearance we compared the rates of degradation of LC3-II in the different cells by immunoblot (LC3-flux assay), even though we were aware that neural-specific changes could be underestimated in this type of analysis, as DAn represent only a fraction of the cells present in whole-culture lysates.
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Federal H.I.V. prevention funds cannot be used for needle exchange programs, even though we are aware that needle exchange programs could save hundreds of lives each year.
Though we are aware that more data need to be collected, this work might be relevant for the introduction of a new preventive protocol.
The choice of Kalman filter does not affect the reliability of our solution, even though we are aware that better alternatives could be used, especially when dealing with the problem of data association [2, 29, 30].
Though we are aware that various problems still have to be solved (such as intermittent carriers and possible false negatives, the duration of decolonization efficacy and the appearance of resistance to treatment) and that more data need to be collected to reinforce statistical analysis, we consider this prophylactic approach to be promising and worth being introduced into daily surgical practice.
In our study, as no formal mental tests were performed, we used the term self-perceived memory loss though we are aware that we may have included persons with cognitive impairment in our group.
We draw on the distinction between research and treatment, even though we are aware that in translational contexts the distinction is sometimes blurred, and when we refer to research activities we include clinical research.
Therefore, we feel that it is justified to use a simplified introgression process at the local population level, even though we are aware that the within-population dynamics are in fact much more complex.
IHC was performed as per standard protocols [ 7] on tissues embedded into FFPE blocks mentioned above, as per standard practice though we are aware that this slice of tissue may not represent the whole tumor.
Always, though, we are aware of her knowing that standing up and staying sane are merely provisional; she could topple over at any second.
The overall impression of "Perpetual Dawn," nonetheless, is too much of a mild muchness, even though at every point we're aware that we're watching the work of a master.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com