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In the closely related connexin 32 protein (Cx32), the substitution of Cys64, which forms the disulfide bridge with Cys168 (the analogous of Cys169 in Cx26), with a serine generates a mutant protein that fails to form gap junction channels, even though it is correctly targeted to the cell membrane (36).
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For example, we observed two cases (entries Rv0519c and Rv3484) where the cleavage site for signal peptidase I was correctly assigned only in the TIGR dataset, even though the region containing it is correctly annotated on both datasets.
Make sure it is correctly.
Has it been correctly applied?
If you do it correctly though, it's fairly painless, and you can truly build your business this way.
Sampling equipment may not perform well in practice even though it is designed correctly.
If the mature peptide provides retrograde information to the neuron on the ability of the peripheral cell to be activated by the neuron, then supplementing it may mislead the organism that the system is functioning correctly even though it is not.
When used correctly this method can be informative [ 112], though it is sensitive to a number of factors and can be prone to errors [ 113].
You cuddle with your cat and stay wrapped in your blanket shivering even though it's 90 degrees outside because your body can't regulate your vitals correctly.
Though it's actually a decent UI and works very well, it just never took off for Microsoft correctly.
[During the procedure] I check the checklist: the nurse had (at some point before the end of the operation) filled the entire thing in, including "specimen is correctly labelled"—even though the specimen-to-be was still inside the patient.
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