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Though endogenous metals present in working solutions may interfere with DAN, the interference may be masked by the addition of 10 mM of EDTA, which we tested here (250 μL; tested ranged from 1 to 100 mM).
Mitchell et al. [10] showed that synthetic miRNAs are immediately degraded in serum even though endogenous circulating miRNAs are stably expressed in serum.
This likely reflected the fact that by stage 54, LGR5 expression was already very high in the limb (Fig. 3B, C) even though endogenous T3 level was still quite low [28].
Recent work demonstrates that even though endogenous fat synthesis is reduced in pept-1 mutants, these worms accumulate high levels of fat due to accelerated uptake of dietary fatty acids [32].
As previously, L35 and L56 transgenic lines tissues were used to maintain the genetic background even though endogenous miRs were quantified.
Though endogenous TAp63 proteins are barely detectable in somatic cells, overexpression of TAp63 proteins promotes cell cycle arrest and apoptotic cell death via activating p53 pro-apoptotic targets, such as Puma, Bax and Noxa.
Similar(51)
Exo, exogenous; Endo, endogenous.
Nuccitelli, R. Measuring endogenous electric fields.
Though rickettsial endogenous endophthalmitis has been reported [11], bilaterality in 9 out of 10 cases, an interval of 2 to 4 weeks between the systemic and ocular presentation and better response to 'steroid-only' treatment in 3 patients in our study points towards an immune-driven process.
In Brassica or Arabidopsis allopolyploid hybrids, underdominant rRNA genes (the set of rRNA genes that is subject to silencing) transfected into protoplasts are transcribed even though their endogenous, chromosomal counterparts are silenced [17], [18].
Note that mature miR-181a and miR-181c have similar expression patterns during thymocyte development [9], [10], though the endogenous levels of miR-181c is about four to five times lower than that of miR-181a in the corresponding thymocytes, indicating that they are processed in thymocytes and may play roles in normal T cell development (Fig. S4).
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