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In the Anastrepha branch, species of the more ancient intrageneric taxonomic groups, such as A. serpentina, A. grandis and A. bistrigata (but not A. striata), lie in sub-branches distinct from those grouping the eight species belonging to the fraterculus taxonomic group.
To analyze mechanisms of evolution of the genes encoding ferrichrome synthetase proteins in more detail, those enzymes grouping with C. heterostrophus NPS2 and those grouping with A. nidulans SidC in phylogenetic analyses of the complete A domain dataset (see above) were examined separately.
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