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If those ESCs that are used also have defects that affect their fitness, then it is possible that the tetraploid cells will persist for longer and, therefore, this will affect the interpretation of the experiment.
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The research group, led by Loewer et al., found that the expression profiles of lincRNAs in iPSCs were similar to those in ESCs but not to those in the somatic cells of origin, such as fibroblasts and hematopoietic stem cells [99].
In recent years, the most breaking advance in cell biology is probably iPSCs, which was first reported by Takahashi and Yamanaka 59 and Yu et al. 60 These cells reprogrammed by using Thomson factors or Yamanaka factors showed morphological characteristics and differentiation abilities (including iPSCs to RPE) similar to those of the ESCs.
Importantly, Epi-iPSΔN-iEsrrb cells reacquired both ESC morphology and levels of Nanog GFP similar to those in ESCs.
Furthermore, levels of Nanog are low in defined conditions but are comparable to those in ESCs for EpiSCs on feeders.
When replication timing differences in OSKM cells are compared to those in ESCs, we observed a conservation of BAF250a-affected domains between ESCs and OSKM cells.
This therefore leads to the conclusion that the cells were cultured cells that possessed expression characteristics that were very similar to those of ESCs.
The transcriptome profiles of INI1 AT/RTs and INI1 ATRT-like tumors were most similar to those of ESCs and NSCs, respectively (Fig. 2c).
We computed the percentage of CpGs in fibroblasts and iPSCs that are significantly different in methylation state from those in ESCs.
Induced pluripotent stem cells (iPSCs) are a type of newly defined stem cell with properties similar to those of ESCs in terms of self-renewal and differentiation.
Despite similar global gene expression and DNA methylation profiles to those of ESCs, iPSCs have been reported to frequently carry substantial genetic and epigenetic abnormalities [ 5, 14].
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