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Samples were rinsed thoroughly prior to imaging.
Immunoprecipitates were washed thoroughly prior to resuspension in 2x Laemmli sample buffer and boiled for 3 minutes.
Lysates were then vortexed thoroughly prior to coating triplicate wells of 96-well Nunc-Immuno™ MaxiSorp™ plates (Fisher Scientific, Leicestershire, UK) with 25 µL of a 1/32 dilution in phosphate buffer saline (PBS) coating buffer of either RSV-A2-infected or mock-infected cell lysates.
This observation, however, does highlight the importance of examining raw data thoroughly prior to association analysis.
All solvents and reagents were purified and dried thoroughly prior to use (see the Supporting Information).
Observers were provided with the observation tool manual and requested to read it thoroughly prior to on-site evaluation.
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The effect of these additives has been thoroughly investigated prior to thin film characterisations.
All the equipment used in the study was thoroughly cleaned prior to the experiments.
The pure ZnO NPs (nZnO) and the FBS-coated formulation (nZnO – FBSdried) were thoroughly characterized prior to use.
It is crucial, however, that the normal keratinocyte stem cell is thoroughly characterized prior to attempting to manipulate its pluripotency.
SCS has the potential to create a paradigm shift in cancer care to precision (personalized) treatment where heterogeneity is thoroughly characterized prior to and during treatment.
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CEO of Professional Science Editing for Scientists @ prosciediting.com