Sentence examples for thoroughly fixed from inspiring English sources

On day 6 cells were washed thoroughly, fixed and permeabilized with methanol (for COX-2) or acetone (for COX-1).

"It was always the plan to get this thoroughly fixed after the Games.

After incubation with NPs for up to 24 h, cells were rinsed thoroughly, fixed, and characterized by darkfield HS imaging.

Co-cultures were washed thoroughly, fixed with 4% PFA/sucrose/PBS, and processed for immunolabelling with anti-GAD65 antibodies, or for electron microscopy.

The bearing plate and dome should be thoroughly fixed together.

Mechanical clocks allowed the days of the industrial revolution to be regularised in new ways; cars changed the landscape and extended the geography of people's lives; the printed book made human knowledge more accessible, more easily built on and more thoroughly examinable, fixing it in bindings onto shelves.

The U.S. Nuclear Regulatory Commission, citing serious concerns about equipment failures at the San Onofre nuclear power plant, has prohibited Southern California Edison from restarting the plant until the problems are thoroughly understood and fixed.

The cells were then thoroughly washed and fixed in 1% paraformaldehyde.

Isolated islets were thoroughly rinsed and then fixed with 4% paraformaldehyde for 10 min, followed by cell permeabilization by exposure to 0.2% Triton X-100 on ice for 10 min. Nonspecific antigens were blocked by 30 min exposure to 5% FBS (blocking buffer).

The antennae were thoroughly washed in PBS and fixed again for 20 min. Fixative was washed away with PBS and antennae pre-hybridized in Hybridization Buffer for 8 hr at 55°C.

The solutions were adsorbed on mica, fixed with Karnovsky fixative and rinsed thoroughly with water for atomic force microscopy (AFM).