By combining the approach of design of experiments (DOE) with subject matter expertise and experience, we were able to understand thoroughly how assay parameters affect the performance of the assay individually and interactively, identify the key assay parameters, define assay operating ranges and finally achieve a robust and sensitive cell-based assay for the detection of NAbs to Conatumumab.
Whatman filter No.1 paper discs were soaked with the extracts and air-dried thoroughly before the assay.
This was the most unexpected finding of our study, and because of its implications for human diet and health, we went on to demonstrate, as thoroughly as the assay allows, that there is also no detectable Ins P6 in human serum, plasma or urine.
We cannot rule out heterogeneity in copy number of some blocks, but this heterogeneity was seen on samples showing wide 95% confidence intervals for the PRT, and suggests that PRT assays thoroughly optimized for the chimpanzee genome are likely to show smaller confidence intervals.
Conditions tested on in organello mtRNA degradation assay were thoroughly tested on the purified protein.
This is reasonable considering that routine pathology analyses only limited a part of the lymph node, whereas the OSNA assay can thoroughly evaluate the entire lymph node.
In brief, cells cultured as described for the MTT assay were thoroughly washed with PBS and incubated in 0.1 ml assay buffer (0.1 M sodium acetate, 0.1% Triton X-100 (vol/vol), 4 mg/ml phosphatase substrate) for 90 minutes.
This is reasonable considering that a single-section histology analyses only a limited part of the lymph node, whereas the OSNA assay can thoroughly evaluate total metastatic volume in a lymph node.
The agreement in detecting HPV infections with HC2, a thoroughly validated clinical screening assay [ 18], was moderate for both CLART and LA, but with no statistically significant differences in detecting high-grade CIN.
