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This was gently mixed on a roller for 10 min at room temperature before automated analysis for leucocyte count, erythrocyte count, Hb concentration, haematocrit, platelet count, mean corpuscular volume, mean corpuscular Hb, mean corpuscular Hb concentration, number and percentage of lymphocytes, monocytes and granulocytes (ABC, Scil Vet).
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The best way to handle this is gently.
The pronounced frequency of FCRS investigated in this paper was gently influenced by its layer system, and gradually changed from 20 Hz to 22 Hz as depth increased.
This solution was gently heated and allowed to stir vigorously at ambient temperature for 72 h.
This mixture was gently pipetted until the pellet fully dissolved, typically in 30 seconds.
A second intravenous catheter was placed in a distal arm or hand vein; this arm was gently heated, allowing for sampling of arterialized venous blood.
This infection suspension was gently pipetted up and down using a sterile 5 mL pipette until a uniform suspension was achieved, and the concentration was adjusted to an optical density of 0.3 to 0.5 at 600 nm.
After this period, each mouse was gently placed inside a clean Plexiglas chamber (42×42×30 cm; Accuscan Instruments, Columbus, OH) for 30 minutes to assess locomotor activity in a new environment.
After this the knee-joint was gently flexed and extended until palpable lysis of adhesions was completed and a ROM of 120°-130° wachievedved.
To this end, the colony surface was gently rubbed with a sterile T-spatula, and the conidial suspension was filtered through sterile glass wool and maintained at 4 °C until further analysis.
"It was gently handled".
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