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To assume that tragedy has lost viability is to forget that this viability was seriously questioned by the first Western philosopher to address himself to the problem.
Using flow cytometry (Fig. 6a), we determined that ~72 77% of the cells in culture were viable, regardless of the presence or absence of ECM coating, and that this viability was maintained over time (assessed at 15 DIV and 30 DIV).
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This viability is determined herein using a linear combination metric, which combines efficiency and CO2 emissions.
This viability is commonly assayed by dye exclusion techniques where cells are incubated with a dilute solution of dye which only enters dead cells.
In this combination, the viability was reduced to 0%.
After 15 hours at this concentration, the viability was decreased by 38% in HOCl fibroblasts and by 14% in PBS fibroblasts (P < 0.001; Figure 2a).
Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control.
Underlying this high viability was the low (1.7%) cell-to-droplet volume fraction we used during encapsulation process.
After this procedure viability was tested using trypan blue.
Numbers of DMSO-exposed control cells were taken to be 100% viable and from this, percentage viability was calculated for exposed cells.
This decrease in viability was not higher than that recorded for the cell-free AuNP-only controls in the interference studies (11% to 24% decrease).
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