As an example of the abundance of this type of transcript in mosquito sialotranscriptomes, only one transcript out of 503 clones coded for apyrase in the C. quinquefasciatus sialotranscriptome [ 5], while 99 out of 4,066 were found in a similarly made library from An. gambiae, and 66 out of 4,232 ESTs were found in the Ae. aegypti sialotranscriptome [ 4].
Splicing alterations in CLL specimens can alter the type of transcripts and thereby function of a large number of cellular proteins that may provide the cell with survival advantage.
a LENGTH and DEPTH are the length and the depth of contigs, respectively, and C/NC is a dummy variable for the type of transcript (protein-coding (coded with 1) vs non-coding transcript (coded with 0).
a LENGTH and DEPTH are the length and the depth of contigs, respectively, and C/NC is a dummy variable for the type of transcript (protein-coding (coded as 1) vs non-coding transcript (coded as 0)).
We then searched systematically for this type of RNAseq only transcripts, i.e. that would satisfy both criteria, a perfect match on genomic sequences and no good match with genome-predicted transcripts (<95% sequence identity), indicating that they were arising from other loci.
The expressions of these types of transcripts or genes were confirmed by reverse transcription polymerase chain reaction (RT-PCR).
The existence of these types of transcripts is supported by and consistent with EST data.
We did not find this type of "bi-functional" chimeric transcripts in Rattus norvegicus or Mus musculus.
Since the model search for a DGM can involve dependencies among transcripts, this type of model can avoid this potential pitfall.
Indeed, transcript profiling of brat k06028 mutants identified transcripts of this type of which misregulation appears to be associated with malignant growth.
