Unfortunately, such structurally-based alignment algorithms are too expensive computationally for this type of high-throughput analysis.
Improved screening techniques will facilitate this type of high-throughput investigation in zebrafish, adding to existing mammalian models as we advance our understanding of cardiovascular disease.
This type of high-throughput, high-fidelity data will constitute a vast wealth of connectivity information and will contribute to building a detailed understanding of neural circuits at microscopic scales.
To acquire and analyze this type of data with higher throughput, we decided to make use of novel methods in imaging and analysis.
Molecular Devices integrates this type of software into high-throughput screening platforms, so scientists can quickly scan thousands of spheroids growing in varied culture conditions.
Several groups have recently adapted this type of approach for high-throughput RNAi screening to identify new regulators of cell migration.
Overall, this type of rapid, scalable, and high throughput method for probing levels of multiple phosphoproteins from single samples could provide new insights into functional and coordinated aspects of disease altered signaling, revealing underlying drivers of cancer progression and informative therapeutic opportunities.
This case study shows that by adopting some practical techniques, it is possible to use the GPU's computational power to perform this type of processing and achieve significant throughputs, which until recently could be obtained only by developing VLSI dedicated microelectronics systems.
Mixture-model analysis can formally account for metabolite 'missingness' due to absence or undetectability, but software for this type of analysis in the high-throughput setting is limited.
MRM-based quantitation can also be multiplexed to analyze and quantify hundreds of proteins per run, increasing the throughput of this type of assay and making it fast enough for clinical applications.
