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However, the overexpression of Nrd1 did not completely rescue the ts− phenotype of hip3-1 mutant (Fig. 3A), suggesting that this ts− phenotype is not caused simply by the inhibition of the transcriptional repression of meiosis-specific genes.
This TS structure supports a concerted mechanism.
The mild phenotype in tac-1 lf) tac-1 lf due to incouldete loss of function of this ts allele.
This Ts has been reported to be caused by UV-radiation and the deamination of methylated C [ 26].
Overall, this indicates that screens of this ts collection should be relatively comprehensive with respect to pathways because the alleles represent all annotated biological pathways among essential genes.
By increasing the number of alleles and improving the annotation, this ts collection will serve as a community resource for probing new aspects of biology for essential yeast genes.
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