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This transcript presents 4 exons, as for the CG33329 predicted transcript, but the third exon is longer in the cloned cDNA.
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Since it has been reported that GAPDH expression is altered in neurodegenerative diseases [ 11], we evaluated several endogenous controls by qRT-PCR in the frontal cortex regions and RPLP0 was chosen because this transcript presented overall low Ct values and lowest Ct variation (unpublished data).
The transcript presents a rare view of La Cosa Nostra's cocaine trafficking operation and provides an indication that key members of the organization feared detection by law enforcement, prosecutors said.
This transcript is present in both P. aegeria oocytes and ovarioles, but its role in oogenesis has not been described in the literature.
Expression analysis of HMG-CoA reductase indicates that this transcript is present in high levels in both germinated urediniospores and appressoria, but in relative low levels in urediniospores prior to germination.
This means that, although the transcripts present differences at sequence level, many of them can be associated to a single locus revealing the high level of heterozygosity of the C. sinensis genome and/or the high frequency of alternative transcripts in the orange genome.
Also consistent with the ESTcalc estimate that we have tagged all of the transcripts present in this sample, our unigene accumulation curve shows that the rate of new unigene detection for this cDNA library has declined to the point that additional sequencing is unlikely to detect new genes, but may however serve to condense and join non-overlapping contigs in our assembly.
This might indicate that the greater the amount of aHIF transcript present, the more HIF-1α mRNA is spliced.
Interestingly, the RP703 transcripts did not reach this termination site but exhibited a diffuse pattern of termination reminiscent of the transcripts presented in Figure 1 (data not shown).
Instead of capturing transcript molecules by molecular hybridisation, as on microarrays, RNA-seq directly sequences the transcripts present in a sample.
Transcript abundance was normalized to human HNF1A(A) transcript present in each tissue.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com