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Unlike in the array experiment where N-myc levels modestly recovered at day 7 due to exhaustion of Tet, N-myc levels in this subsequent experiment were reduced nearly 200-fold consistently at days 3, 5, and 7.
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Because of this, subsequent experiments required a classical genetics approach.
Considering this, the subsequent experiments were carried out to elucidate the impact of the combined therapy on tumour perfusion, oxygenation and metabolic microenvironment, in comparison to the effects of HT alone, since such effects may play a key role in the observed inhibition of tumour growth.
To avoid possible stress from social isolation and allow normal social development in this and the subsequent experiments, fish were kept in pairs in 4 litre tanks from birth to the time of the test.
The only criterion to include subjects in this and the subsequent experiments was their willingness to participate in the tasks.
For this reason, in the subsequent experiments, the highest concentration was no longer tested.
In the subsequent experiments, this polymer composition was characterized with different techniques.
A 10-nM dose of T3 in the tail culture media was consistently sufficient to induce tail fin resorption; thus, we used this dose throughout the subsequent experiments.
This issue is of particular importance for the subsequent experiments in this paper, which use mice that still retain one allele of TCF8 because null mice are perinatal lethal [25].
However, no significant effects involving this factor were found in this, or either subsequent experiment.
For this and subsequent experiments, all participants read the appropriate consent form and indicated their consent to participate.
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