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The destination vector used in this study was constructed by modifying the pTT3 vector [ 7].

The chronic comorbidity system in this study was constructed by selecting ICD-9-CM codes suggestive of chronic disease within separate organ systems.

Second, the model in this study was constructed by using relatively short-term data for a 6-year period, which could have made the results less reliable.

The framework of this study was constructed by mainly referring to related theoretical ideas, such as the dual-factor model and the Technology Acceptance Model, and was used to discuss factors that influence a physician's usage of medical information technologies.

The model examined in this study was constructed by several members of the study team over a period of several months, based on their clinical observation that as patients approached death, there were changing patterns in the patients' ESAS symptom profiles.

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Unless otherwise indicated, plasmids used in this study were constructed by Gateway technology (GW; Invitrogen) following the instructions of the manufacturer.

The humanization and maturation libraries in this study were constructed by PCR assembly of overlapping oligonucleotides containing variable nucleotides at key positions to generate mutations at specific sites.

The microarray chips for this study were constructed by Agilent Technologies.

Collectively, and as depicted in the Figure (in which phylogenetic alignment trees for all 3 genes tested in this study were constructed by using reference sequences from representative members of the genera Anaplasma, Ehrlichia, and Neorickettsia), the genetic findings in this study support infection of horses in Nicaragua with a novel Ehrlichia species.

The data set used in this historic (or retrospective) cohort study was constructed by linking the Total Population Register, the Multi-Generation Register, the Swedish Inpatient Register, the Swedish Outpatient Care Register and the Swedish Cause of Death Register, which were provided by Statistics Sweden and the National Board of Health and Welfare.

The burst database employed in the present study was constructed by searching MSHA accident report narratives for terms such as "burst", "bump" and "bounce".

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