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For safety concerns, a replication-deficient adenoviral vector (ΔE1/ΔE3) was used in this study (Supplementary Materials and Methods) (He et al., 1998).
In this study, supplementary methods were developed for e.g. sampling of intact bryozoan limestone cores and for saturation and contamination of the cores with cis-DCE.
Among these, 80 sequences match DNA sequences from species used in this study (Supplementary Table S2).
Table S2 shows the primers sets used for this study (supplementary methods).
Of sequences containing errors in the primers 313 of 377 (83.0%) matched species used in this study (Supplementary Table S3).
Many genes associated with photosynthesis, carbon fixation processes, and chlorophyll synthesis were suppressed by RDV infection in this study (Supplementary Figure S6).
In total, 466 B. pertussis isolates were included in this study (Supplementary Table S1).
We classified these sequences as NCFSs in this study (Supplementary Material, Table S1).
31 This association could, however, not be confirmed in this study (supplementary table 1).
We also included these sequences on our array, and categorized them as NCFSs in this study (Supplementary Material, Table S1).
Smaducin-6 was synthesized at a purity of more than 95% to be used in this study (Supplementary Fig S4A).
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